Thursday, April 28, 2005

Posttranscriptional Control of Gene Expression in Yeast -- McCarthy 62 (4): 1492 -- Microbiology and Molecular Biology Reviews

1. Does only mature mRNAs leave the nucleus?
It is generally agreed that, with very few exceptions, only mature mRNAs (bound by RNA-binding proteins [mRNPs]) leave the nucleus .

2. Does mRNA transportation associate with cytoskeleton?
The fact that mRNA is observed associated with microtubules and actin filaments suggests that these components of the cytoskeleton may be responsible for (selective) mRNA transport. Recent evidence also indicates that at least one yeast mRNA (ASH1) becomes localized within the cell by virtue of its association with the cytoskeleton (535).

3. How long is poly(A) tail in mRNA of Yeast?
At the 3' end, the mRNA carries a poly(A) tail (initially 60 to 90 adenylate residues in yeast.

Friday, April 22, 2005

genomic regions that do not encode proteins is also transcribed

Transcription by RNA polymerase II in Saccharomyces cerevisiae and in humans is widespread, even in genomic regions that do not encode proteins.

From : Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene.
PMID: 15175754

Coupled transcription and translation within nuclei of mammalian cells

What a wierd thing!

Science. 2001 Aug 10;293(5532):1139-42.
PMID: 11423616

Monday, April 04, 2005

iHOP - Information Hyperlinked over Proteins - Abstract Viewer