mRNA and protein abundance in a cell
mRNA abundance range from 0.001 to 200 copies per cell:
1. Analysis of 60,633 transcripts in yeast revealed 4,665 genes, with expression levels ranging from 0.3 to over 200 transcripts per cell.[PMID: 9008165].
2. Steady-state transcript levels encoded by all 65 genes on the left arm of chromosome III and 185 transcription factor genes in yeast are quantitated using kinetically monitored, reverse transcriptase-initiated PCR (kRT-PCR). [PMID: 11882647].
Protein abundance range from 50 to 1,000,000 copies per cell.
1. The abundance of proteins ranges from fewer than 50 to more than 10(6) molecules per cell in yeast. Many of these molecules, including essential proteins and most transcription factors, are present at levels that are not readily detectable by other proteomic techniques nor predictable by mRNA levels or codon bias measurements.[PMID: 14562106]
1. Analysis of 60,633 transcripts in yeast revealed 4,665 genes, with expression levels ranging from 0.3 to over 200 transcripts per cell.[PMID: 9008165].
2. Steady-state transcript levels encoded by all 65 genes on the left arm of chromosome III and 185 transcription factor genes in yeast are quantitated using kinetically monitored, reverse transcriptase-initiated PCR (kRT-PCR). [PMID: 11882647].
Protein abundance range from 50 to 1,000,000 copies per cell.
1. The abundance of proteins ranges from fewer than 50 to more than 10(6) molecules per cell in yeast. Many of these molecules, including essential proteins and most transcription factors, are present at levels that are not readily detectable by other proteomic techniques nor predictable by mRNA levels or codon bias measurements.[PMID: 14562106]
posted by Yiming Zhou at 3:46 PM
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